Fluorimetric determination of hydrogen peroxide production by the haemocytes of the wax moth Galleria mellonella L. (Lepidoptera: Pyralidae)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F10%3A00045625" target="_blank" >RIV/00216224:14310/10:00045625 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Fluorimetric determination of hydrogen peroxide production by the haemocytes of the wax moth Galleria mellonella L. (Lepidoptera: Pyralidae)

Original language description

The aim of this work was to investigate whether haemocytes of Galleria mellonella (Lepidoptera: Pyralidae) larvae can produce reactive oxygen species (ROS) in comparison to human blood phagocytes. The production of ROS was measured first with the use ofluminol - enhanced chemiluminescence of unstimulated and stimulated (four activators with different mode of action) haemolymph or isolated haemocytes. However, spontaneous as well as the activated ROS generation remained at the background level. In subsequent experiments an ultrasensitive fluorescence method using Amplex Red reagent to detect hydrogen peroxide (H2O2) was tested. After optimization, Amplex Red was successfully used for determination of H2O2 production in both unstimulated and stimulatedhaemocytes. To determine the role of pH and ions during measurement, several diluent solutions were tested. We have shown that Ca2+ and Mg2+ ions are less important for the reaction in insects as compared to mammalian cells.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

ED - Physiology

OECD FORD branch

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Project

<a href="/en/project/GP206%2F09%2FP470" target="_blank" >GP206/09/P470: The influence of the eicosanoid biosynthesis inhibitors to immunity of the wax moth Galleria mellonella</a><br>

Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

European Journal of Entomology

ISSN

1210-5759

e-ISSN

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Volume of the periodical

X

Issue of the periodical within the volume

X

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

6

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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