Identification of Staphylococcus spp. using (GTG)5-PCR fingerprinting

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F10%3A00045696" target="_blank" >RIV/00216224:14310/10:00045696 - isvavai.cz</a>
Alternative codes found
RIV/75010330:_____/10:00009031
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Identification of Staphylococcus spp. using (GTG)5-PCR fingerprinting
Original language description
A group of 212 type and reference strains deposited in the Czech Collection of Microorganisms (Brno, Czech Republic) and covering 41 Staphylococcus species comprising 21 subspecies was characterised using rep-PCR fingerprinting with the (GTG)5 primer inorder to evaluate this method for identification of staphylococci. All strains were typeable using the (GTG)5 primer and generated PCR products ranging from 200 to 4500 bp. Numerical analysis of the obtained fingerprints revealed (sub)speciesspecific clustering corresponding with the taxonomic position of analysed strains. Taxonomic position of selected strains representing the (sub)species that were distributed over multiple rep-PCR clusters was verified and confirmed by the partial rpoB gene sequencing.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Project
<a href="/en/project/GA310%2F09%2F0459" target="_blank" >GA310/09/0459: Role of bacteriophages in horizontal transfer of virulence and drug resistance genes in Staphylococcus aureus</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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