Characterization of staphylococcal type strains by whole-cell protein analysis and (GTG)5-PCR fingerprinting.

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F06%3A00021871" target="_blank" >RIV/00216224:14310/06:00021871 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Characterization of staphylococcal type strains by whole-cell protein analysis and (GTG)5-PCR fingerprinting.
Original language description
A group of 47 type strains representing all validly described staphylococcal species and subspecies was characterized by whole-cell protein analysis by using sodium dodecylsulfate-polyacrylamide gel electrophoresis and rep-PCR fingerprinting with the (GTG)5 primer. All strains were well typeable and revealed unique fingerprints by both methods. The individual staphylococcal type strains were differentiated on the level of 74 % using the protein analysis, with exception of novobiocin-resistant species, and on the level of 68 % by using the (GTG)5-PCR fingerprinting. Generally, all subspecies of the individual species showed close protein profiles that were grouped into individual clusters. Similarly, but with a few exceptions, the individual subspecieswere separated into the distinct clusters using the (GTG)5-PCR fingerprinting. No clear correlation between the clustering obtained by both methods and the phylogenetic relationships of the analysed species was observed.
Czech name
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Czech description
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Publication year
2006
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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