Random mutagenesis as a tool for improving properties of PA-IIL lectin from Pseudomonas aeruginosa

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F10%3A00057485" target="_blank" >RIV/00216224:14310/10:00057485 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Random mutagenesis as a tool for improving properties of PA-IIL lectin from Pseudomonas aeruginosa
Original language description
Random mutagenesis is the most useful approach in directed evolution, widely used in protein engineering for producing proteins with altered or improved properties. Random mutagenesis includes a lot of methods used to create diverse mutant libraries. Oneof these methods is error-prone PCR. This technique introduces nucleotide changes during PCR due to the use of error-prone DNA polymerase and mutational reaction conditions. LecB is the target gene we have modified via random mutagenesis. This gene codes PA-IIL lectin from Pseudomonas aeruginosa. This lectin is well-characterized and it is indisputable that it displays an unusually high affinity for monosaccharides widely occurring as terminal residues in cell surface glycoconjugates highly present incystic fibrosis mucins. The random mutagenesis could help us to create a protein with interestingly modified properties towards monospecificity.
Czech name
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Czech description
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Project
<a href="/en/project/GD301%2F09%2FH004" target="_blank" >GD301/09/H004: Molecular and structural biology of selected antitumor drugs. From mechanistic studies to chemotherapy of tumors</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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