Methods of directed evolution of PA-IIL lectin

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F10%3A00044241" target="_blank" >RIV/00216224:14310/10:00044241 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Methods of directed evolution of PA-IIL lectin
Original language description
Directed evolution is a powerful tool widely used in protein engineering for producing proteins with altered or improved properties. This approach includes a lot of methods used to create diverse mutant libraries and to screen these libraries. In our work two mutation techniques are being used. The first one is random mutagenesis by error-prone PCR (which introduces nucleotide changes during PCR due to the use of error-prone DNA polymerase and reaction conditions). The second one is targeted-random mutagenesis (which employs mixture of special primers carried random base substitutions on selected site). LecB, the gene coding PA-IIL lectin from bacterium Pseudomonas aeruginosa, was chosen as a target gene to be modified via random mutagenesis. This soluble protein is well-characterized and it is indisputable that it displays an unusually high affinity for monosaccharides widely occurring as terminal residues in cell surface glycoconjugates highly present in cystic fibrosis mucins.
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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