Random mutagenesis of lectins and high-throughput screening of mutant libraries

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F11%3A00049899" target="_blank" >RIV/00216224:14740/11:00049899 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Random mutagenesis of lectins and high-throughput screening of mutant libraries
Original language description
Random mutagenesis is often used in protein engineering for producing proteins with altered or improved properties. Random mutagenesis includes a lot of methods used to create diverse mutant libraries and to screen these libraries. In our work two methodical directions are being applied. Error-prone PCR is based on error-prone DNA polymerase which introduces nucleotide changes during extension of amplified DNA. Mutational reaction conditions can support even higher number of random substitutions. Targeted-random mutagenesis is being performed by mixture of special oligonucleotide primers carried random base substitutions on selected site(s). An effective high-throughput screening must be employed while large mutant libraries are produced by random mutagenesis techniques. Currently, methods of high-throughput screening have been optimized to maximize of automation of this process. Several approaches simplifying the protein mixture for binding studies were applied.
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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