Antibacterial methods based on bioluminescent bacteria
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F11%3A00049709" target="_blank" >RIV/00216224:14310/11:00049709 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Antibacterial methods based on bioluminescent bacteria
Original language description
Bioluminescent bacteria are common in salt water environment, but there is the only one genus of soil bacteria with natural bioluminescence ? Photorhabdus sp. These symbiotic bacteria reside the gut of entomopathogenic nematodes which are obligate insectparasites with the increasing importance as biological control agent. Isolated Photorhabdus luminescens was used separately for determination of pathogenity to insects. Except P. luminescens the artificial bioluminescent bacterium was used - geneticallymodified Escherichia coli K12 that carries Photorhabdus genes for bioluminescence. Both of these G- bacteria can be used for antibacterial assays based on their bioluminescence ability. Bioluminescence reaction is mediated by bacterial enzyme luciferasewhich catalyses the oxidation of long-chain aldehyde (substrate) and reduces flavin mononucleotide with emission of light. This emission can be immediately measured by the luminometer.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
EC - Immunology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GP206%2F09%2FP470" target="_blank" >GP206/09/P470: The influence of the eicosanoid biosynthesis inhibitors to immunity of the wax moth Galleria mellonella</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů