Bioluminescent assay for evaluating antimicrobial activity in insect haemolymph

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F14%3A00073253" target="_blank" >RIV/00216224:14310/14:00073253 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.14411/eje.2014.045" target="_blank" >http://dx.doi.org/10.14411/eje.2014.045</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.14411/eje.2014.045" target="_blank" >10.14411/eje.2014.045</a>

Result language

angličtina

Original language name

Bioluminescent assay for evaluating antimicrobial activity in insect haemolymph

Original language description

We describe an antibacterial assay based on bioluminescence of two Gram negative bacteria, Photorhabdus luminescens and transformed Escherichia coli, which can be used as a real-time measurement of antibacterial activity in insect haemolymph. This methodis based on the production of the bioluminescence signal depending on the viability of bacterial cells. We observed a significant rapid dose-dependent decrease in bioluminescence using both bacterial species, and Bombyx mori or Galleria mellonella haemolymph, which was confirmed by the decrease in bacterial viability determined by plating. The humoral origin of the antibacterial activity observed in whole haemolymph was confirmed for haemolymph plasma without haemocytes. Antibacterial activity directedagainst Gram negative bacteria was recorded in unaffected insect larvae as well as after septic injury; increased antibacterial activity of haemolymph was detected in the latter case confirming the inducibility of antimicrobial agents.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EC - Immunology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

European Journal of Entomology

ISSN

1210-5759

e-ISSN

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Volume of the periodical

111

Issue of the periodical within the volume

3

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

6

Pages from-to

335-340

UT code for WoS article

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EID of the result in the Scopus database

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