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Post-translational modifications regulate signalling by Ror1

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F11%3A00050218" target="_blank" >RIV/00216224:14310/11:00050218 - isvavai.cz</a>

  • Alternative codes found

    RIV/68081707:_____/11:00440915

  • Result on the web

    <a href="http://dx.doi.org/10.1111/j.1748-1716.2011.02306.x" target="_blank" >http://dx.doi.org/10.1111/j.1748-1716.2011.02306.x</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1111/j.1748-1716.2011.02306.x" target="_blank" >10.1111/j.1748-1716.2011.02306.x</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Post-translational modifications regulate signalling by Ror1

  • Original language description

    Ror1 (receptor tyrosine kinase-like orphan receptor)is highly upregulated in B cells of patients with chronic lymphocytic leukaemia (CLL). Ror1 acts as the Wnt receptor in the non-canonical Wnt pathway. We demonstrate that Ror1 is extensively modified byN-linked glycosylation. Glycosylation produces several variants of Ror1 with electrophoretic migration of approx. 100, 115 and 130 kDa. Inhibition of glycosylation interferes with cell surface localization of the 130-kDa variant of Ror1 and prevents Ror1-induced formation of filopodia. Moreover, we show that 130-kDa Ror1 is mono-ubiquitinated. Furthermore, individual CLL patients show striking differences in the electrophoretic migration of Ror1, which correspond to the level of glycosylation. Our datashow that Ror1 undergoes complex post-translational modifications by glycosylation and mono-ubiquitination. These modifications regulate Ror1 localization and signalling, and are highly variable among individual CLL patients.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EB - Genetics and molecular biology

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2011

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Acta Physiologica

  • ISSN

    1748-1708

  • e-ISSN

  • Volume of the periodical

    203

  • Issue of the periodical within the volume

    3

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    12

  • Pages from-to

    351-362

  • UT code for WoS article

    000296061800002

  • EID of the result in the Scopus database