Structural and Functional Characterization of the Wnt Inhibitor APC Membrane Recruitment 1 (Amer1)
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F11%3A00054246" target="_blank" >RIV/00216224:14310/11:00054246 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1074/jbc.M111.224881" target="_blank" >http://dx.doi.org/10.1074/jbc.M111.224881</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1074/jbc.M111.224881" target="_blank" >10.1074/jbc.M111.224881</a>
Alternative languages
Result language
angličtina
Original language name
Structural and Functional Characterization of the Wnt Inhibitor APC Membrane Recruitment 1 (Amer1)
Original language description
Amer1/WTX binds to the tumor suppressor adenomatous polyposis coli and acts as an inhibitor of Wnt signaling by inducing beta catenin degradation. We show here that Amer1 directly interacts with the armadillo repeats of beta catenin via a domain consisting of repeated arginine-glutamic acid-alanine (REA) motifs, and that Amer1 assembles the beta catenin destruction complex at the plasma membrane by recruiting beta catenin, adenomatous polyposis coli, and Axin/Conductin. Deletion or specific mutations ofthe membrane binding domain of Amer1 abolish its membrane localization and abrogate negative control of Wnt signaling, which can be restored by artificial targeting of Amer1 to the plasma membrane. In line, a natural splice variant of Amer1 lacking theplasma membrane localization domain is deficient for Wnt inhibition. Knockdown of Amer1 leads to the activation of Wnt target genes, preferentially in dense compared with sparse cell cultures, suggesting that Amer1 function is regulated b
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
JOURNAL OF BIOLOGICAL CHEMISTRY
ISSN
0021-9258
e-ISSN
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Volume of the periodical
286
Issue of the periodical within the volume
22
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
19204-19214
UT code for WoS article
000291027700003
EID of the result in the Scopus database
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