Measurements of genomic GC content in plant genomes with flow cytometry: a test for reliability
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F12%3A00057231" target="_blank" >RIV/00216224:14310/12:00057231 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1111/j.1469-8137.2011.03942.x" target="_blank" >http://dx.doi.org/10.1111/j.1469-8137.2011.03942.x</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/j.1469-8137.2011.03942.x" target="_blank" >10.1111/j.1469-8137.2011.03942.x</a>
Alternative languages
Result language
angličtina
Original language name
Measurements of genomic GC content in plant genomes with flow cytometry: a test for reliability
Original language description
Knowledge of phylogenetic pattern and biological relevance of the base composition of large eukaryotic genomes (including plants) is poor. With the use of flow cytometry (FCM), the available data on the GC content in plants have nearly doubled in the last decade. However, skepticism exists concerning the reliability of the method because of uncertainty in some input parameters. Here, we tested the reliability of FCM for estimating the GC content by comparison with the biochemical method of DNA melting temperature analysis (TMA). We conducted measurements in 14 plant species with a maximum currently known GC content range (33.6?47.5%; FCM). We also compared the estimations of the GC content by FCM with genomic sequences in 11 Oryza species. FCM and TMAdata exhibited a high degree of correspondence that remained stable over the relatively wide range of binding lengths (3.39?4.09) assumed for the base-specific dye used.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EF - Botany
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
New Phytologist
ISSN
0028-646X
e-ISSN
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Volume of the periodical
193
Issue of the periodical within the volume
2
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
513-521
UT code for WoS article
000298591800024
EID of the result in the Scopus database
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