An improved method for nematode infection assays in Drosophila larvae
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F12%3A00057254" target="_blank" >RIV/00216224:14310/12:00057254 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.4161/fly.19553" target="_blank" >http://dx.doi.org/10.4161/fly.19553</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.4161/fly.19553" target="_blank" >10.4161/fly.19553</a>
Alternative languages
Result language
angličtina
Original language name
An improved method for nematode infection assays in Drosophila larvae
Original language description
The infective juveniles (IJs) of entomopathogenic nematodes (EPNs) seek out host insects and release their symbiotic bacteria into their body cavity causing septicaemia, which eventually leads to host death. The interaction between EPNs and their hosts are only partially understood, in particular the host immune responses appears to involve pathways other than phagocytosis and the canonical transcriptional induction pathways. These pathways are genetically tractable and include for example clotting factors and lipid mediators. The aim of this study was to optimize the nematode infections in Drosophila melanogaster larvae, a well-studied and genetically tractable model organism. Here we show that two nematode species namely Steinernema feltiae and Heterorhabditis bacteriophora display different infectivity towards Drosophila larvae with the latter being less pathogenic. The effects of supporting media and IJ dosage on the mortality of the hosts were assessed and optimized.
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EC - Immunology
OECD FORD branch
—
Result continuities
Project
<a href="/en/project/GP206%2F09%2FP470" target="_blank" >GP206/09/P470: The influence of the eicosanoid biosynthesis inhibitors to immunity of the wax moth Galleria mellonella</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Fly
ISSN
1933-6934
e-ISSN
—
Volume of the periodical
6
Issue of the periodical within the volume
2
Country of publishing house
US - UNITED STATES
Number of pages
5
Pages from-to
75-79
UT code for WoS article
—
EID of the result in the Scopus database
—