Physiological and proteomic approaches to evaluate the role of sterol binding in elicitin-induced resistance

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F12%3A00057274" target="_blank" >RIV/00216224:14310/12:00057274 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1093/jxb/err427" target="_blank" >http://dx.doi.org/10.1093/jxb/err427</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/jxb/err427" target="_blank" >10.1093/jxb/err427</a>

Result language
angličtina
Original language name
Physiological and proteomic approaches to evaluate the role of sterol binding in elicitin-induced resistance
Original language description
Cryptogein is a proteinaceous elicitor secreted by Phytophthora cryptogea that can induce resistance to P. parasitica in tobacco plants. On the basis of previous computer modelling experiments, by site-directed mutagenesis a series of cryptogein variantswas prepared with altered abilities to bind sterols, phospholipids or both. The sterol binding and phospholipid transfer activities corresponded well with the previously reported structural data. Induction of the synthesis of reactive oxygen species (ROS) in tobacco cells in suspension and proteomic analysis of intercellular fluid changes in tobacco leaves triggered by these mutant proteins were not proportional to their ability to bind or transfer sterols and phospholipids. However, changes in the intercellular proteome corresponded to transcription levels of defence genes and resistance to P. parasitica and structureprediction of mutants did not reveal any significant changes in protein structure.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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