Construction of Cryptogein Mutants, a Proteinaceous Elicitor from Phytophthora, with Altered Abilities To Induce a Defense Reaction in Tobacco Cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F05%3A00012448" target="_blank" >RIV/00216224:14310/05:00012448 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Construction of Cryptogein Mutants, a Proteinaceous Elicitor from Phytophthora, with Altered Abilities To Induce a Defense Reaction in Tobacco Cells

Original language description

We prepared a series of cryptogein mutants, an elicitor from Phytophthora cryptogea, with altered abilities to bind sterols and fatty acids. The induction of the early events, i.e., synthesis of active oxygen species and pH changes, in suspension tobaccocells by these mutated proteins was proportional to their ability to bind sterols but not fatty acids. Although the cryptogein-sterol complex was suggested to be a form triggering a defense reaction in tobacco, some proteins unable to bind sterols induced the synthesis of active oxygen species and pH changes. The modeling experiments showed that conformational changes after the introduction of bulky residues into the loop of cryptogein resemble those induced by sterol binding. These changes may be necessary for the ability to trigger the early events by elicitins. However, the ability to stimulate necrosis in suspension tobacco cells and the expression of defense proteins in tobacco plants were linked neither to the lipid binding capac

Czech name

Konstrukce mutantu kryptogeinu, elicitoru houby Phytophthora, s modifikovanými vlastnostmi idnukce obranné reakce u bunek tabaku

Czech description

Konstrukce mutantu kryptogeinu, elicitoru houby Phytophthora

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

—

Project

<a href="/en/project/GA522%2F02%2F0925" target="_blank" >GA522/02/0925: Modeling of the structure and interaction of elicitors of the fungus Phytophthora</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biochemistry

ISSN

0006-2960

e-ISSN

—

Volume of the periodical

44

Issue of the periodical within the volume

17

Country of publishing house

US - UNITED STATES

Number of pages

13

Pages from-to

6565

UT code for WoS article

—

EID of the result in the Scopus database

—