Unwinding of synthetic replication and recombination substrates by Srs2
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F12%3A00057534" target="_blank" >RIV/00216224:14310/12:00057534 - isvavai.cz</a>
Alternative codes found
RIV/00159816:_____/12:#0000964
Result on the web
<a href="http://dx.doi.org/10.1016/j.dnarep.2012.05.007" target="_blank" >http://dx.doi.org/10.1016/j.dnarep.2012.05.007</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.dnarep.2012.05.007" target="_blank" >10.1016/j.dnarep.2012.05.007</a>
Alternative languages
Result language
angličtina
Original language name
Unwinding of synthetic replication and recombination substrates by Srs2
Original language description
The budding yeast Srs2 protein possesses 3 to 5 DNA helicase activity and channels untimely recombination to post-replication repair by removing Rad51 from ssDNA. However, it also promotes recombination via a synthesis-dependent strand-annealing pathway(SDSA). Furthermore, at the replication fork, Srs2 is required for fork progression and prevents the instability of trinucleotide repeats. To better understand the multiple roles of the Srs2 helicase during these processes, we analysed the ability of Srs2 to bind and unwind various DNA substrates that mimic structures present during DNA replication and recombination. While leading or lagging strands were efficiently unwound, the presence of ssDNA binding protein RPA presented an obstacle for Srs2 translocation. We also tested the preferred directionality of unwinding of various substrates and studied the effect of Rad51 and Mre11 proteins on Srs2 helicase activity.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
DNA Repair
ISSN
1568-7864
e-ISSN
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Volume of the periodical
11
Issue of the periodical within the volume
10
Country of publishing house
GB - UNITED KINGDOM
Number of pages
10
Pages from-to
789-798
UT code for WoS article
000310761100002
EID of the result in the Scopus database
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