Efficient transfer of antibiotic resistance plasmids by transduction within methicillin-resistant Staphylococcus aureus USA300 clone
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F12%3A00057819" target="_blank" >RIV/00216224:14310/12:00057819 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1111/j.1574-6968.2012.02589.x" target="_blank" >http://dx.doi.org/10.1111/j.1574-6968.2012.02589.x</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/j.1574-6968.2012.02589.x" target="_blank" >10.1111/j.1574-6968.2012.02589.x</a>
Alternative languages
Result language
angličtina
Original language name
Efficient transfer of antibiotic resistance plasmids by transduction within methicillin-resistant Staphylococcus aureus USA300 clone
Original language description
The epidemic community-associated methicillin-resistant clone Staphylococcus aureus USA300 is a major source of skin and soft tissue infections and involves strains with a diverse set of resistance genes. In this study, we report efficient transduction of penicillinase and tetracycline resistance plasmids by bacteriophages phi80alpha and phiJB between clinical isolates belonging to the USA300 clone. High transduction frequencies (10-5 to 10-6 CFU/PFU) were observed using phages propagated on donor strains as well as prophages induced from donors by ultraviolet light. Quantitative real-time PCR was employed to detect penicillinase plasmids in transducing phage particles and determine the ratio of transducing particles in phage lysates to infectious phage particles (determined as approximately 1:1700). Successful transfer of plasmids between strains in USA300 clone proves transduction is an effective mechanism for spreading plasmids within the clone.
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
—
Result continuities
Project
<a href="/en/project/GA310%2F09%2F0459" target="_blank" >GA310/09/0459: Role of bacteriophages in horizontal transfer of virulence and drug resistance genes in Staphylococcus aureus</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
FEMS Microbiology Letters
ISSN
0378-1097
e-ISSN
—
Volume of the periodical
332
Issue of the periodical within the volume
2
Country of publishing house
GB - UNITED KINGDOM
Number of pages
7
Pages from-to
146-152
UT code for WoS article
000305334300008
EID of the result in the Scopus database
—