Efficient plasmid transduction to Staphylococcus aureus strains insensitive to the lytic action of transducing phage

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F16%3A00088273" target="_blank" >RIV/00216224:14310/16:00088273 - isvavai.cz</a>
Result on the web
<a href="http://femsle.oxfordjournals.org/content/363/19/fnw211" target="_blank" >http://femsle.oxfordjournals.org/content/363/19/fnw211</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/femsle/fnw211" target="_blank" >10.1093/femsle/fnw211</a>

Result language
angličtina
Original language name
Efficient plasmid transduction to Staphylococcus aureus strains insensitive to the lytic action of transducing phage
Original language description
The transduction mediated by bacteriophages is considered to be one of the primary driving forces in horizontal gene transfer in staphylococci, which is crucial to their adaptation and successful evolution. For a transduction to be effective, it is generally accepted that the recipient strain should be susceptible to the transducing phage. In this study, we demonstrate that the plasmid DNAs are effectively transduced into the recipient Staphylococcus aureus strains in spite of their insensitivity to the lytic action of the transducing phage, provided that these phages adsorb effectively to the bacterial cells. The tetracycline and penicillinase plasmids were transduced to insensitive laboratory and clinical strains by bacteriophages phi29, phi52A and phi80alpha as well as by prophage phi53 and naturally occurring prophages induced from donor lysogenic strains. Comparable frequencies of transduction were achieved in both phage-sensitive and phage-insensitive recipient strains.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Project
<a href="/en/project/GP13-05069P" target="_blank" >GP13-05069P: Horizontal transfer of Staphylococcal Cassette Chromosome mec (SCCmec) elements mediated by bacteriophages across the Staphylococcus strains</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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