Expression of hypothetical lectin from pisum sativum-comparison of two eukaryotic expression systems
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F12%3A00057853" target="_blank" >RIV/00216224:14310/12:00057853 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Expression of hypothetical lectin from pisum sativum-comparison of two eukaryotic expression systems
Original language description
We have identified a new putative lectin from Pisum sativum using SDS-PAGE and MS analysis of highly purified pea amine oxidase where it was present as an impurity. This study was aimed at the production of this protein in a eucaryotic expression systemand its structure-function characterization. As production of the native protein was found very low, we have compared two yeast expression systems - Pichia pastoris and Saccharomyces cerevisiae. Advantages/disadvantages of both approaches will be discussed in detail.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA522%2F08%2F0555" target="_blank" >GA522/08/0555: Structure-functional characterization of oxidoreductases acting on nitrogenous regulatory compounds in plants</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů