Conjugation of 5(6)-carboxyfluorescein and 5(6)-carboxynaphthfluorescein with bovine serum albumin and their immobilization for optical pH sensing

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F12%3A00062930" target="_blank" >RIV/00216224:14310/12:00062930 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.snb.2011.09.046" target="_blank" >http://dx.doi.org/10.1016/j.snb.2011.09.046</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.snb.2011.09.046" target="_blank" >10.1016/j.snb.2011.09.046</a>

Result language

angličtina

Original language name

Conjugation of 5(6)-carboxyfluorescein and 5(6)-carboxynaphthfluorescein with bovine serum albumin and their immobilization for optical pH sensing

Original language description

An approach for the immobilization of a pH indicator in optical pH sensors and biosensors was developed. Fluorescent dyes 5(6)-carboxyfluorescein and 5(6)-carboxynaphthofluorescein were conjugated with bovine serum albumin as a non-enzymatic scaffold protein and the conjugation procedure was optimized. Fluorescent properties, sensitivity to temperature and photostability of conjugates have been studied and characterized into details. The conjugates were immobilized on glass support by: (i) glutaraldehyde crosslinking or (ii) entrapment in sol?gel matrix ORMOCER with subsequent glutaraldehyde cross-linking. The response to pH and leaching potential were evaluated. The sensor layer, based on immobilized conjugates of 5(6)-carboxyfluorescein and bovine serum albumin, displayed rapid response over the pH range 4.0?9.0, making it compatible with a range of applications such as bioprocessing, clinical diagnostics or environmental monitoring.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/ED0001%2F01%2F01" target="_blank" >ED0001/01/01: CETOCOEN</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

SENSORS AND ACTUATORS B: CHEMICAL

ISSN

0925-4005

e-ISSN

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Volume of the periodical

161

Issue of the periodical within the volume

1

Country of publishing house

CH - SWITZERLAND

Number of pages

7

Pages from-to

93-99

UT code for WoS article

000301549400007

EID of the result in the Scopus database

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