Retargeting a maize b-glucosidase to the vacuole ? Evidence from intact plants that zeatin-O-glucoside is stored in the vacuole
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F12%3A00064517" target="_blank" >RIV/00216224:14310/12:00064517 - isvavai.cz</a>
Result on the web
<a href="http://www.sciencedirect.com/science/article/pii/S0031942212001458" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0031942212001458</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.phytochem.2012.03.012" target="_blank" >10.1016/j.phytochem.2012.03.012</a>
Alternative languages
Result language
angličtina
Original language name
Retargeting a maize b-glucosidase to the vacuole ? Evidence from intact plants that zeatin-O-glucoside is stored in the vacuole
Original language description
Cytokinin (CK) activity is regulated by the complex interplay of their metabolism, transport, stability and cellular/tissue localization. O-glucosides of zeatin-type CKs are postulated to be storage and/or transport forms. Active CK levels are determinedin part by their differential distribution of CK metabolites across different subcellular compartments. We have previously shown that overexpressing chloroplast-localized Zm-p60.1, a maize b-glucosidase capable of releasing active cytokinins from theirO- and N3-glucosides, perturbs CK homeostasis in transgenic tobacco. We obtained tobacco (Nicotiana tabacum L., cv Petit Havana SR1) plants overexpressing a recombinant Zm-p60.1 that is targeted to the vacuole. The protein is correctly processed and localized to the vacuole. When grown on medium containing exogenous zeatin, transgenic seedlings rapidly accumulate fresh weight due to ectopic growths at the base of the hypocotyl.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/LC06034" target="_blank" >LC06034: Regulation of morphogenesis of plant cells and organs</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Phytochemistry
ISSN
0031-9422
e-ISSN
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Volume of the periodical
79
Issue of the periodical within the volume
06
Country of publishing house
GB - UNITED KINGDOM
Number of pages
11
Pages from-to
67-77
UT code for WoS article
000307031800006
EID of the result in the Scopus database
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