Retargeting a maize beta-glucosidase to the vacuole -- Evidence from intact plants that zeatin-O-glucoside is stored in the vacuole

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F12%3A00188697" target="_blank" >RIV/62156489:43210/12:00188697 - isvavai.cz</a>

Alternative codes found

RIV/68081707:_____/12:00381585 RIV/61389030:_____/12:00381585

Result on the web

<a href="http://dx.doi.org/10.1016/j.phytochem.2012.03.012" target="_blank" >http://dx.doi.org/10.1016/j.phytochem.2012.03.012</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.phytochem.2012.03.012" target="_blank" >10.1016/j.phytochem.2012.03.012</a>

Result language

angličtina

Original language name

Retargeting a maize beta-glucosidase to the vacuole -- Evidence from intact plants that zeatin-O-glucoside is stored in the vacuole

Original language description

Cytokinin (CK) activity is regulated by the complex interplay of their metabolism, transport, stability and cellular/tissue localization. O-glucosides of zeatin-type CKs are postulated to be storage and/or transport forms. Active CK levels are determinedin part by their differential distribution of CK metabolites across different subcellular compartments. We have previously shown that overexpressing chloroplast-localized Zm-p60.1, a maize beta-glucosidase capable of releasing active cytokinins from their O- and N3-glucosides, perturbs CK homeostasis in transgenic tobacco. We obtained tobacco (Nicotiana tabacum L., cv Petit Havana SR1) plants overexpressing a recombinant Zm-p60.1 that is targeted to the vacuole. The protein is correctly processed and localized to the vacuole. When grown on medium containing exogenous zeatin, transgenic seedlings rapidly accumulate fresh weight due to ectopic growths at the base of the hypocotyl. The presence of the enzyme in these ectopic structures is

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Phytochemistry

ISSN

0031-9422

e-ISSN

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Volume of the periodical

79

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

12

Pages from-to

66-77

UT code for WoS article

307031800006

EID of the result in the Scopus database

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