Fish MHC genes - quality vs quantity
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F13%3A00067000" target="_blank" >RIV/00216224:14310/13:00067000 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Fish MHC genes - quality vs quantity
Original language description
Even if the role, variability and evolutionary mechanism of selection of genes of major histocompatibility complex (MHC genes) has been proved for several times to be very important in immunoecological studies and in investigation of associations betweenmultiple parasites and host immunocompetence in cyprinids, the question of quantitative expression of these genes stays still unanswered. Our research reveals that not only ?quality? (genomic variability or allelic richness) but also ?quantity? of thesegenes (active gene expression and its level) may play an important role in host-parasite interactions and may be useful for evolutionary and population studies. By utilizing two modern approaches (high resolution melting analysis ? HRM and quantitativePCR ? qPCR) we obtained notable primary results that could supplement or possibly stand in the analysis by classical methods in MHC research (CE-SSCP, automatic sequencing based on Sanger technology or 454 clonal pyrosequencing).
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GBP505%2F12%2FG112" target="_blank" >GBP505/12/G112: ECIP - European Centre of Ichtyoparasitology</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů