BIOPHYSICAL STUDY OF THE INTERACTION OF ZINC IONS WITH DNA

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F13%3A00068522" target="_blank" >RIV/00216224:14310/13:00068522 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
BIOPHYSICAL STUDY OF THE INTERACTION OF ZINC IONS WITH DNA
Original language description
We focused interactions of zinc ions and DNA in this study. Interactions were monitored using UV/VIS spectrophotometry and gel electrophoresis. Firstly, we isolated and amplified fragment of DNA of 498 bp. DNA was subsequently incubated with Zn(II) ionsfor 60 min at 25 C. After incubation, samples were dialyzed and analysed immediately. In this way, DNA was converted into a metal bound DNA (M-DNA). Interaction of Zn(II) with DNA showed a change of the absorption spectrum (190-350 nm) and a decrease inthe M-DNA melting temperature. Spectrophotometric (UV/VIS) analysis showed that increasing concentrations of Zn(II) led to an increase in absorbance at 200 nm and a decrease in absorbance at 260 nm. Application of 5.5 microM Zn(II) caused a decrease in the melting temperature (Tm) for about 7.5 C in average in comparison with control (75.5 +- 3 C). The lowest melting temperature (60.5 +- 2.5 C) was observed after application of 33 microM Zn(II).
Czech name
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Czech description
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Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
XIII. Pracovní setkání fyzikálních chemiků a elektrochemiků, sborník
ISBN
9788073757571
ISSN
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e-ISSN
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Number of pages
2
Pages from-to
66-67
Publisher name
Mendelova univerzita v Brně
Place of publication
Brno
Event location
Brno
Event date
Jan 1, 2013
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
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