HPLC profiles of polyphenolic compounds and estimation of antioxidant potential in sea buckthorn plant parts
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F13%3A00070150" target="_blank" >RIV/00216224:14310/13:00070150 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
HPLC profiles of polyphenolic compounds and estimation of antioxidant potential in sea buckthorn plant parts
Original language description
Predominant medical effect of many plants and herbs is related to the presence of polyphenolic secondary plant metabolites commonly known as flavonoids. Different classes of flavonoids are synthesized as a response to the ambient stimuli (climate, stress, mold attacks etc.) and accumulate in various cells and plant parts. The antioxidant potential and selected flavonoids content in sea buckthorn (Hippophaë Rhamnoides L.) plant part materials (stems, leaves and fruits) has been investigated during one vegetative cycle. New RP-HPLC method using gradient elution for determination of 10 polyphenols, namely catechin, epicatechin, rutin, quercetin, quercitrin, kaempferol, gallic acid, p-coumaric acid, caffeic acid and ferulic acid has been developed. Significant part of method development included optimization of extraction step for sample matrix removal. As the most suitable, liquid-liquid extraction of methanolic plant solutions using diethylether was found.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů