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HPLC profiles of polyphenolic compounds and estimation of antioxidant potential in sea buckthorn plant parts

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F13%3A00070150" target="_blank" >RIV/00216224:14310/13:00070150 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    HPLC profiles of polyphenolic compounds and estimation of antioxidant potential in sea buckthorn plant parts

  • Original language description

    Predominant medical effect of many plants and herbs is related to the presence of polyphenolic secondary plant metabolites commonly known as flavonoids. Different classes of flavonoids are synthesized as a response to the ambient stimuli (climate, stress, mold attacks etc.) and accumulate in various cells and plant parts. The antioxidant potential and selected flavonoids content in sea buckthorn (Hippophaë Rhamnoides L.) plant part materials (stems, leaves and fruits) has been investigated during one vegetative cycle. New RP-HPLC method using gradient elution for determination of 10 polyphenols, namely catechin, epicatechin, rutin, quercetin, quercitrin, kaempferol, gallic acid, p-coumaric acid, caffeic acid and ferulic acid has been developed. Significant part of method development included optimization of extraction step for sample matrix removal. As the most suitable, liquid-liquid extraction of methanolic plant solutions using diethylether was found.

  • Czech name

  • Czech description

Classification

  • Type

    O - Miscellaneous

  • CEP classification

    CB - Analytical chemistry, separation

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    S - Specificky vyzkum na vysokych skolach

Others

  • Publication year

    2013

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů