Investigation of interaction between magnetic silica particles and lambda phage DNA fragment
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F13%3A00072703" target="_blank" >RIV/00216224:14310/13:00072703 - isvavai.cz</a>
Alternative codes found
RIV/62156489:43210/13:00213505 RIV/00216305:26620/13:PU105678
Result on the web
<a href="http://dx.doi.org/10.1016/j.jpba.2013.07.039" target="_blank" >http://dx.doi.org/10.1016/j.jpba.2013.07.039</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jpba.2013.07.039" target="_blank" >10.1016/j.jpba.2013.07.039</a>
Alternative languages
Result language
angličtina
Original language name
Investigation of interaction between magnetic silica particles and lambda phage DNA fragment
Original language description
Nucleic acids belong to the most important molecules and therefore the understanding of their properties, function and behavior is crucial. Even though a range of analytical and biochemical methods have been developed for this purpose, one common step isessential for all of them isolation of the nucleic acid from the from complex sample matrix. The use of magnetic particles for the separation of nucleic acids has many advantages over other isolation methods. In this study, an isolation procedure for extraction of DNA was optimized. Each step of the isolation process including washing, immobilization and elution was optimized and therefore the efficiency was increased from 1.7% to 28.7% and the total time was shortened from 75 to 30 min comparing to the previously described method. Quantification of the particular parameter influence was performed by square-wave voltammetry using hanging drop mercury electrode.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CF - Physical chemistry and theoretical chemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Pharmaceutical and Biomedical Analysis
ISSN
0731-7085
e-ISSN
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Volume of the periodical
86
Issue of the periodical within the volume
1
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
8
Pages from-to
65-72
UT code for WoS article
000325671200007
EID of the result in the Scopus database
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