Problematic barcoding in flatworms: A case-study on monogeneans and rhabdocoels (Platyhelminthes)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F13%3A00094005" target="_blank" >RIV/00216224:14310/13:00094005 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.3897/zookeys.365.5776" target="_blank" >http://dx.doi.org/10.3897/zookeys.365.5776</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3897/zookeys.365.5776" target="_blank" >10.3897/zookeys.365.5776</a>

Result language

angličtina

Original language name

Problematic barcoding in flatworms: A case-study on monogeneans and rhabdocoels (Platyhelminthes)

Original language description

Some taxonomic groups are less amenable to mitochondrial DNA barcoding than others. Due to the paucity of molecular information of understudied groups and the huge molecular diversity within flatworms, primer design has been hampered. Indeed, all attempts to develop universal flatworm-specific COI markers have failed so far. We demonstrate how high molecular variability and contamination problems limit the possibilities for barcoding using standard COI-based protocols in flatworms. As a consequence, molecular identification methods often rely on other widely applicable markers. In the case of Monogenea, a very diverse group of platyhelminth parasites, and Rhabdocoela, representing one-fourth of all free-living flatworm taxa, this has led to a relatively high availability of nuclear ITS and 18S/28S rDNA sequences on GenBank. In a comparison of the effectiveness in species assignment we conclude that mitochondrial and nuclear ribosomal markers perform equally well.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EG - Zoology

OECD FORD branch

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Project

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Continuities

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

ZOOKEYS

ISSN

1313-2989

e-ISSN

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Volume of the periodical

2013

Issue of the periodical within the volume

365

Country of publishing house

BG - BULGARIA

Number of pages

25

Pages from-to

355-379

UT code for WoS article

000329206600021

EID of the result in the Scopus database

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