Disheveled regulates precoupling of heterotrimeric G proteins to Frizzled 6
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F14%3A00073587" target="_blank" >RIV/00216224:14310/14:00073587 - isvavai.cz</a>
Alternative codes found
RIV/68081707:_____/14:00428653
Result on the web
<a href="http://dx.doi.org/10.1096/fj.13-246363" target="_blank" >http://dx.doi.org/10.1096/fj.13-246363</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1096/fj.13-246363" target="_blank" >10.1096/fj.13-246363</a>
Alternative languages
Result language
angličtina
Original language name
Disheveled regulates precoupling of heterotrimeric G proteins to Frizzled 6
Original language description
Frizzleds (FZDs) are classified as G-protein-coupling receptors, but how signals are initiated and specified through heterotrimeric G proteins is unknown. FZD6 regulates convergent extension movements, and its C-terminal Arg511Cys mutation causes nail dysplasia in humans. We investigated the functional relationship between FZD6, Disheveled (DVL), and heterotrimeric G proteins. Live cell imaging combined with fluorescence recovery after photobleaching (FRAP) revealed that inactive human FZD6 precouples to GALPHAi1 and GALPHAq but not to GALPHAoA,GALPHAs, and GALPHA12 proteins. G-protein coupling is measured as a 10-20% reduction in the mobile fraction of fluorescently tagged G proteins on chemical receptor surface cross-linking. The FZD6 Arg511Cys mutation is incapable of G-protein precoupling, even though it still binds DVL. Using both FRAP and Förster resonance energy transfer (FRET) technology, we showed that the FZD6-GALPHAi1 and FZD-GALPHAq complexes dissociate on WNT-5A stimulatio
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
FASEB JOURNAL
ISSN
0892-6638
e-ISSN
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Volume of the periodical
28
Issue of the periodical within the volume
5
Country of publishing house
US - UNITED STATES
Number of pages
13
Pages from-to
2293-2305
UT code for WoS article
000335336000031
EID of the result in the Scopus database
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