Do variability and expression level of fish MHC genes play crucial role in parasite load and host immunocompetence?
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F14%3A00074146" target="_blank" >RIV/00216224:14310/14:00074146 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Do variability and expression level of fish MHC genes play crucial role in parasite load and host immunocompetence?
Original language description
We have confirmed extremely high level of allelic diversity in several fish genera by classical genotyping. We used two basic approaches for this purpose. First, the older, was CE-SSCP (Capillary Electrophoresis-Single Stranded Conformational Polymorphism) of PCR amplicon clones combined with automatic sequencing based on Sanger technology (ABI 3130 Genetic Analyzer). The second, modern, was 454 clonal pyrosequencing of heterozygous amplicons (454 GS FLX platform with Titanium chemistry). Recently, using CFX96 real time PCR system, we test also the expression levels of fish MHC genes (DAB1 and DAB3) by quantitative real-time PCR (qPCR) and we launched a new approach for MHC genes variability tests - high resolution melting analysis (HRM), too. After completing "HRM allele profile database" and finish analyses of qPCR we will be exploring associations with parasite load in chosen Cyprinid species.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GBP505%2F12%2FG112" target="_blank" >GBP505/12/G112: ECIP - European Centre of Ichtyoparasitology</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
3rd Workshop of European Centre of Ichthyoparasitology
ISBN
9788021074927
ISSN
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e-ISSN
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Number of pages
2
Pages from-to
10-11
Publisher name
MUNI Press
Place of publication
Brno
Event location
Hotel Skalní mlýn, Blansko
Event date
Jan 1, 2014
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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