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A Pseudomonas putida Strain Genetically Engineered for 1,2,3-Trichloropropane Bioremediation.

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F14%3A00074202" target="_blank" >RIV/00216224:14310/14:00074202 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.1128/AEM.01620-14" target="_blank" >http://dx.doi.org/10.1128/AEM.01620-14</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1128/AEM.01620-14" target="_blank" >10.1128/AEM.01620-14</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    A Pseudomonas putida Strain Genetically Engineered for 1,2,3-Trichloropropane Bioremediation.

  • Original language description

    1,2,3-Trichloropropane (TCP) is a toxic compound that is recalcitrant to biodegradation in the environment and attempts to isolate TCP-degrading organisms using enrichment cultivation have failed. A potential biodegradation pathway starts with hydrolyticdehalogenation to 2,3 dichloro-1-propanol (DCP), followed by oxidative metabolism. To obtain a practically applicable TCP-degrading organism, we introduced an engineered haloalkane dehalogenase with improved TCP degradation activity into the DCP-degrading bacterium Pseudomonas putida MC4. For this, the dehalogenase gene (dhaA31) was cloned behind the constitutive dhlA promoter and introduced into the genome of strain MC4 using a transposon delivery system. The transposon-located antibiotic resistance marker was subsequently removed using a resolvase step. Growth of the resulting engineered bacterium P. putida MC4-5222 on TCP was indeed observed, and all organic chlorine was released as chloride.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CE - Biochemistry

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GAP503%2F12%2F0572" target="_blank" >GAP503/12/0572: Construction of a synthetic metabolic pathway for degradation of important environmental pollutant using protein and metabolic engineering</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2014

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Applied and Environmental Microbiology

  • ISSN

    0099-2240

  • e-ISSN

  • Volume of the periodical

    80

  • Issue of the periodical within the volume

    17

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    10

  • Pages from-to

    5467-5476

  • UT code for WoS article

    000341486500032

  • EID of the result in the Scopus database