Crystallization and Crystallographic Analysis of the Rhodococcus rhodochrous NCIMB 13064 DhaA Mutant DhaA31 and its Complex with 1,2,3-Trichloropropane

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F11%3A00049447" target="_blank" >RIV/00216224:14310/11:00049447 - isvavai.cz</a>

Alternative codes found

RIV/67179843:_____/11:00372340 RIV/60076658:12520/11:43882929

Result on the web

<a href="http://dx.doi.org/10.1107/S1744309111001254" target="_blank" >http://dx.doi.org/10.1107/S1744309111001254</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1107/S1744309111001254" target="_blank" >10.1107/S1744309111001254</a>

Result language

angličtina

Original language name

Crystallization and Crystallographic Analysis of the Rhodococcus rhodochrous NCIMB 13064 DhaA Mutant DhaA31 and its Complex with 1,2,3-Trichloropropane

Original language description

Haloalkane dehalogenases hydrolyze carbon-halogen bonds in a wide range of halogenated aliphatic compounds. The potential use of haloalkane dehalogenases in bioremediation applications has stimulated intensive investigation of these enzymes and their engineering. The mutant DhaA31 was constructed to degrade the anthropogenic compound 1,2,3-trichloropropane (TCP) using a new strategy. This strategy enhances activity towards TCP by decreasing the accessibility of the active site to water molecules, thereby promoting formation of the activated complex. The structure of DhaA31 will help in understanding the structure-function relationships involved in the improved dehalogenation of TCP. The mutant protein DhaA31 was crystallized by the sitting-drop vapour-diffusion technique and crystals of DhaA31 in complex with TCP were obtained using soaking experiments. Both crystals belonged to the triclinic space group P1.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Crystallographica F

ISSN

1744-3091

e-ISSN

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Volume of the periodical

67

Issue of the periodical within the volume

67

Country of publishing house

GB - UNITED KINGDOM

Number of pages

4

Pages from-to

397-400

UT code for WoS article

000288225100022

EID of the result in the Scopus database

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