Crystallographic Analysis of 1,2,3?Trichloropropane Biodegradation by Haloalkane Dehalogenase DhaA31
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F14%3A00074213" target="_blank" >RIV/00216224:14310/14:00074213 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1107/S1399004713026254" target="_blank" >http://dx.doi.org/10.1107/S1399004713026254</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1107/S1399004713026254" target="_blank" >10.1107/S1399004713026254</a>
Alternative languages
Result language
angličtina
Original language name
Crystallographic Analysis of 1,2,3?Trichloropropane Biodegradation by Haloalkane Dehalogenase DhaA31
Original language description
Haloalkane dehalogenases catalyze the hydrolytic cleavage of carbon-halogen bonds, which is a key step in aerobic mineralization of many environmental pollutants. One important pollutant is the toxic and anthropogenic compound 1,2,3-trichloropropane (TCP). Rational design was combined with saturation mutagenesis to obtain the haloalkane dehalogenase variant DhaA31, which displays an increased catalytic activity towards TCP. Here we report the 1.31 A crystal structure of substrate-free DhaA31, the 1.26 Astructure of DhaA31 in a complex with TCP, and the 1.85 A wild-type DhaA structure. Crystals of the enzyme-substrate complex were successfully obtained by adding volatile TCP to the reservoir at crystallization at room temperature and pH 6.4. Comparisonof the substrate-free structure with the DhaA31 enzyme-substrate complex reveals that the nucleophilic Asp106 changes its conformation from an inactive to an active state during the catalytic cycle.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GAP207%2F12%2F0775" target="_blank" >GAP207/12/0775: Structure-functional Relationships of Haloalkane Dehalogenases</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Acta Crystallographica D
ISSN
0907-4449
e-ISSN
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Volume of the periodical
70
Issue of the periodical within the volume
february
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
209-217
UT code for WoS article
000331554500001
EID of the result in the Scopus database
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