Crystallographic analysis of 1,2,3-trichloropropane biodegradation by the haloalkane dehalogenase DhaA31
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F14%3A43886872" target="_blank" >RIV/60076658:12310/14:43886872 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12520/14:43886872
Result on the web
<a href="http://loschmidt.chemi.muni.cz/peg/wp-content/uploads/2014/02/acta14.pdf" target="_blank" >http://loschmidt.chemi.muni.cz/peg/wp-content/uploads/2014/02/acta14.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1107/S1399004713026254" target="_blank" >10.1107/S1399004713026254</a>
Alternative languages
Result language
angličtina
Original language name
Crystallographic analysis of 1,2,3-trichloropropane biodegradation by the haloalkane dehalogenase DhaA31
Original language description
Haloalkane dehalogenases catalyze the hydrolytic cleavage of carbon-halogen bonds, which is a key step in the aerobic mineralization of many environmental pollutants. One important pollutant is the toxic and anthropogenic compound 1,2,3-trichloropropane(TCP). Rational design was combined with saturation mutagenesis to obtain the haloalkane dehalogenase variant DhaA31, which displays an increased catalytic activity towards TCP. Here, the 1.31 angstrom resolution crystal structure of substrate-free DhaA31, the 1.26 angstrom resolution structure of DhaA31 in complex with TCP and the 1.95 angstrom resolution structure of wild-type DhaA are reported. Crystals of the enzyme-substrate complex were successfully obtained by adding volatile TCP to the reservoirafter crystallization at pH 6.5 and room temperature. Comparison of the substrate-free structure with that of the DhaA31 enzyme-substrate complex reveals that the nucleophilic Asp106 changes its conformation from an inactive to an active
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
—
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Acta Crystallographica Section D - Biological Crystallography
ISSN
1399-0047
e-ISSN
—
Volume of the periodical
70
Issue of the periodical within the volume
Neuveden
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
209-217
UT code for WoS article
000331554500001
EID of the result in the Scopus database
—