Maximizing the Efficiency of Multi-enzyme Process by Stoichiometry Optimization.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F14%3A00074203" target="_blank" >RIV/00216224:14310/14:00074203 - isvavai.cz</a>
Alternative codes found
RIV/00159816:_____/14:00061171 RIV/00216305:26230/14:PU122819
Result on the web
<a href="http://dx.doi.org/10.1002/cbic.201402265" target="_blank" >http://dx.doi.org/10.1002/cbic.201402265</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/cbic.201402265" target="_blank" >10.1002/cbic.201402265</a>
Alternative languages
Result language
angličtina
Original language name
Maximizing the Efficiency of Multi-enzyme Process by Stoichiometry Optimization.
Original language description
Multi-enzyme processes represent an important area of biocatalysis. Their efficiency can be enhanced by optimization of biocatalysts? stoichiometry. Here we present a workflow for maximizing the efficiency of a three-enzyme system catalysing a five-stepchemical conversion. Kinetic models of pathways featuring either wild-type or engineered enzymes were built and the enzyme stoichiometry of each pathway was optimized. Mathematical modelling and one-pot multi-enzyme laboratory experiments provided detailed insights into pathway dynamics, enabled the selection of suitable engineered enzyme and afforded high efficiency while minimizing biocatalyst loadings. The optimizing of stoichiometry in a pathway with engineered enzyme reduced the total biocatalyst load by an impressive 56 %. Our new workflow represents a broadly applicable strategy for optimizing multi-enzyme processes.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
ChemBioChem
ISSN
1439-4227
e-ISSN
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Volume of the periodical
15
Issue of the periodical within the volume
13
Country of publishing house
DE - GERMANY
Number of pages
5
Pages from-to
1891-1895
UT code for WoS article
000341586100007
EID of the result in the Scopus database
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