Computer-Assisted Engineering of Synthetic Pathway for Biodegradation of Toxic Persistent Pollutant.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F14%3A00074211" target="_blank" >RIV/00216224:14310/14:00074211 - isvavai.cz</a>
Alternative codes found
RIV/00216305:26230/14:PU122818
Result on the web
<a href="http://dx.doi.org/10.1021/sb400147n" target="_blank" >http://dx.doi.org/10.1021/sb400147n</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/sb400147n" target="_blank" >10.1021/sb400147n</a>
Alternative languages
Result language
angličtina
Original language name
Computer-Assisted Engineering of Synthetic Pathway for Biodegradation of Toxic Persistent Pollutant.
Original language description
Anthropogenic halogenated compounds were unknown to Nature until the industrial revolution, and microorganisms have not had sufficient time to evolve enzymes for their degradation. The lack of efficient enzymes and natural pathways can be addressed through a combination of protein and metabolic engineering. We have assembled a synthetic route for conversion of the highly toxic and recalcitrant 1,2,3-trichloropropane to glycerol in Escherichia coli, and used it for a systematic study of pathway bottlenecks. Optimal ratios of enzymes for the maximal production of glycerol, and minimal toxicity of metabolites were predicted using a mathematical model. The strains containing the expected optimal ratios of enzymes were constructed and characterized for their viability and degradation efficiency. Excellent agreement between predicted and experimental data was observed.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
ACS Synthetic Biology
ISSN
2161-5063
e-ISSN
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Volume of the periodical
3
Issue of the periodical within the volume
3
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
172-181
UT code for WoS article
000333476900009
EID of the result in the Scopus database
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