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Computer-Assisted Engineering of the Synthetic Pathway for Biodegradation of a Toxic Persistent Pollutant

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F14%3A00061001" target="_blank" >RIV/00159816:_____/14:00061001 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.1021/sb400147n" target="_blank" >http://dx.doi.org/10.1021/sb400147n</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1021/sb400147n" target="_blank" >10.1021/sb400147n</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Computer-Assisted Engineering of the Synthetic Pathway for Biodegradation of a Toxic Persistent Pollutant

  • Original language description

    Anthropogenic halogenated compounds were unknown to nature until the industrial revolution, and microorganisms have not had sufficient time to evolve enzymes for their degradation. The lack of efficient enzymes and natural pathways can be addressed through a combination of protein and metabolic engineering. We have assembled a synthetic route for conversion of the highly toxic and recalcitrant 1,2,3-trichloropropane to glycerol in Escherichia coli, and used it for a systematic study of pathway bottlenecks. Optimal ratios of enzymes for the maximal production of glycerol, and minimal toxicity of metabolites were predicted using a mathematical model. The strains containing the expected optimal ratios of enzymes were constructed and characterized for their viability and degradation efficiency. Excellent agreement between predicted and experimental data was observed. The validated model was used to quantitatively describe the kinetic limitations of currently available enzyme variants and p

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EB - Genetics and molecular biology

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/ED1.100%2F02%2F0123" target="_blank" >ED1.100/02/0123: St. Anne´s University Hospital Brno - International Clinical Research Center (FNUSA-ICRC)</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2014

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    ACS Synthetic Biology

  • ISSN

    2161-5063

  • e-ISSN

  • Volume of the periodical

    3

  • Issue of the periodical within the volume

    3

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    10

  • Pages from-to

    172-181

  • UT code for WoS article

    000333476900009

  • EID of the result in the Scopus database