Comparison of various capillary electrophoretic approaches for the study of drug?protein interaction with emphasis on minimal consumption of protein sample and possibility of automation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F15%3A00080618" target="_blank" >RIV/00216224:14310/15:00080618 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1002/jssc.201400914" target="_blank" >http://dx.doi.org/10.1002/jssc.201400914</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.201400914" target="_blank" >10.1002/jssc.201400914</a>

Result language

angličtina

Original language name

Comparison of various capillary electrophoretic approaches for the study of drug?protein interaction with emphasis on minimal consumption of protein sample and possibility of automation

Original language description

The binding ability of a drug to plasma proteins influences the pharmacokinetics of a drug. As a result, it is a very important issue in new drug development. In this study, affinity capillary electrophoresis, capillary electrophoresis with frontal analysis, and Hummel Dreyer methods with internal and external calibration were used to study the affinity between bovine serum albumin and salicylic acid. The binding constant was measured by all these approaches including the equilibrium dialysis, which isconsidered to be a reference method. The comparison of results and other considerations showed the best electrophoretic approach to be capillary electrophoresis-frontal analysis, which is characterized by the high sample throughput with the possibility of automation, very small quantities of biomacromolecules, simplicity, and a short analysis time. The mechanism of complex formation was then examined by capillary electrophoresis with frontal analysis.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

—

Project

<a href="/en/project/GBP206%2F12%2FG014" target="_blank" >GBP206/12/G014: Center for advanced bioanalytical technologies</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

JOURNAL OF SEPARATION SCIENCE

ISSN

1615-9306

e-ISSN

—

Volume of the periodical

38

Issue of the periodical within the volume

2

Country of publishing house

DE - GERMANY

Number of pages

7

Pages from-to

325-331

UT code for WoS article

000348516600020

EID of the result in the Scopus database

—