Contactless conductivity detector as a tool for improving universality and sensitivity of capillary electrophoresis-frontal analysis: Proof of concept

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F24%3A00135292" target="_blank" >RIV/00216224:14310/24:00135292 - isvavai.cz</a>

Result on the web

<a href="https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/jssc.202300667" target="_blank" >https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/jssc.202300667</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.202300667" target="_blank" >10.1002/jssc.202300667</a>

Result language

angličtina

Original language name

Contactless conductivity detector as a tool for improving universality and sensitivity of capillary electrophoresis-frontal analysis: Proof of concept

Original language description

Drug binding to plasma proteins influences processes such as liberation, adsorption, disposition, metabolism and elimination of drugs, which are thus one of the key steps of a new drug development. As a result, the characterization of drug protein interactions is an essential part of these time- and money-consuming processes. It is important to determine not only the binding strength and the stoichiometry of interaction, but also the binding site of a drug on a protein molecule, because two drugs with the same binding site can mutually affect free drug concentration. Capillary electrophoresis-frontal analysis with mobility shift affinity capillary electrophoresis is one of the most used affinity CE methods for the characterization of these interactions. In this study, a well-known sensitivity problem of most capillary electrophoresis-frontal analyses using UV detection is solved by its combination with contactless conductivity detection, which provided 6-fold lower limits of quantitation and detection. Binding parameters of the human serum albumin salicylic acid model affinity pair were evaluated by this newly developed approach and by the classical approach with UV detection primarily used for their mutual comparison. The results of both approaches agreed well, and are also in agreement with literature data obtained using different techniques.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10600 - Biological sciences

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Separation Science

ISSN

1615-9306

e-ISSN

—

Volume of the periodical

47

Issue of the periodical within the volume

1

Country of publishing house

DE - GERMANY

Number of pages

8

Pages from-to

1-8

UT code for WoS article

001140740000001

EID of the result in the Scopus database

2-s2.0-85182196400