Sensitivity enhancement of capillary electrophoresis‐frontal analysis based method for characterization of drug‐protein interactions using on‐line sample preconcentration

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F23%3A00134152" target="_blank" >RIV/00216224:14310/23:00134152 - isvavai.cz</a>

Result on the web

<a href="https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/full/10.1002/jssc.202300152?campaign=woletoc" target="_blank" >https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/full/10.1002/jssc.202300152?campaign=woletoc</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.202300152" target="_blank" >10.1002/jssc.202300152</a>

Result language

angličtina

Original language name

Sensitivity enhancement of capillary electrophoresis‐frontal analysis based method for characterization of drug‐protein interactions using on‐line sample preconcentration

Original language description

Capillary electrophoresis-frontal analysis is one of the most frequently used approach for the study of plasma protein-drug interactions as a substantial part of the new drug development. However, the capillary electrophoresis-frontal analysis typically combined with ultraviolet visible detection suffers from insufficient concentration sensitivity particularly for substances with limited solubility and low molar absorption coefficient. The sensitivity problem has been solved in this work by its combination with an on-line sample preconcentration. According the knowledge of the authors this combination has never been used to characterize plasma protein-drug binding. It resulted into a fully automated and versatile methodology for the characterization of binding interactions. Further, the validated method minimalizes the experimental errors due to a reduction in the manipulation of samples. Moreover, employing an on-line preconcentration strategy with capillary electrophoresis-frontal analysis using human serum albumin-salicylic acid as a model system improves the drug concentration sensitivity 17 fold compared to the conventional method. The value of binding constant (1.51 ± 0.63)‧104 L/mol obtained by this new capillary electrophoresis-frontal analysis modification is in agreement with the value (1.13 ± 0.28)‧104 L/mol estimated by conventional variant of capillary electrophoresis-frontal analysis without the preconcentration step, as well as with literature data obtained using different techniques.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10600 - Biological sciences

Project

<a href="/en/project/GA19-08358S" target="_blank" >GA19-08358S: New approaches for the study of affinity interaction based on capillary electrophoresis</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Separation Science

ISSN

1615-9306

e-ISSN

—

Volume of the periodical

46

Issue of the periodical within the volume

17

Country of publishing house

DE - GERMANY

Number of pages

8

Pages from-to

1-8

UT code for WoS article

001017663100001

EID of the result in the Scopus database

2-s2.0-85163637022