Simplified protocol for flow cytometry analysis of fluorescently labeled exosomes and microvesicles using dedicated flow cytometer
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F15%3A00080746" target="_blank" >RIV/00216224:14310/15:00080746 - isvavai.cz</a>
Alternative codes found
RIV/65269705:_____/15:00063689 RIV/68081707:_____/15:00472803 RIV/61388971:_____/15:00472803
Result on the web
<a href="http://dx.doi.org/10.3402/jev.v4.25530" target="_blank" >http://dx.doi.org/10.3402/jev.v4.25530</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3402/jev.v4.25530" target="_blank" >10.3402/jev.v4.25530</a>
Alternative languages
Result language
angličtina
Original language name
Simplified protocol for flow cytometry analysis of fluorescently labeled exosomes and microvesicles using dedicated flow cytometer
Original language description
Flow cytometry is a powerful method, which is widely used for high-throughput quantitative and qualitative analysis of cells. However, its straightforward applicability for extracellular vesicles (EVs) and mainly exosomes is hampered by several challenges, reflecting mostly the small size of these vesicles (exosomes: similar to 80-200 nm, microvesicles: similar to 200-1,000 nm), their polydispersity, and low refractive index. The current best and most widely used protocol for beads-free flow cytometry of exosomes uses ultracentrifugation (UC) coupled with floatation in sucrose gradient for their isolation, labeling with lipophilic dye PKH67 and antibodies, and an optimized version of commercial high-end cytometer for analysis. However, this approach requires an experienced flow cytometer operator capable of manual hardware adjustments and calibration of the cytometer.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Extracellular Vesicles
ISSN
2001-3078
e-ISSN
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Volume of the periodical
4
Issue of the periodical within the volume
25530
Country of publishing house
SE - SWEDEN
Number of pages
15
Pages from-to
1-15
UT code for WoS article
000365076000001
EID of the result in the Scopus database
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