Expression of a cysteine peptidase inhibitor from Eudiplozoon nipponicum (Monogenea).

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F15%3A00080924" target="_blank" >RIV/00216224:14310/15:00080924 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Expression of a cysteine peptidase inhibitor from Eudiplozoon nipponicum (Monogenea).
Original language description
Our research is focused primarily on expression and characterization of cystatin produced by platyhelminth Eudiplozoon nipponicum. This blood-feeding ectoparasite of Cyprinus carpio (common carp) is a representative of the family Diplozooidae (Monogenea). Cystatins ? cysteine peptidase inhibitors are produced by a wide range of organisms and belong to bioactive molecules with immunomodulatory and inhibitory properties. The transcriptomic data of E. nipponicum were analyzed for the presence of cystatin sequences. The partial nucleotide sequence was identified and the whole gene sequence was obtained by PCR, 3?/5? RACE PCR and sequencing using cDNA as a template. Gene coding 98 amino acid cystatin of E. nipponicum with predicted molecular weight 10.85 kDa and theoretical pI 6.27 was inserted into pET19b expression vector and the obtained construct was transferred into E. coli competent cells (BL 21 strain). Expression of recombinant cystatin molecule was induced by adding IPTG to the cul
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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