Exacerbation of Substrate Toxicity by IPTG in Escherichia coli BL21(DE3) Carrying a Synthetic Metabolic Pathway

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F15%3A00081393" target="_blank" >RIV/00216224:14310/15:00081393 - isvavai.cz</a>
Alternative codes found
RIV/68081707:_____/15:00456331 RIV/00159816:_____/15:00063782
Result on the web
<a href="http://loschmidt.chemi.muni.cz/peg/wp-content/uploads/2015/12/Dvorak_2015MCF.pdf" target="_blank" >http://loschmidt.chemi.muni.cz/peg/wp-content/uploads/2015/12/Dvorak_2015MCF.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/s12934-015-0393-3" target="_blank" >10.1186/s12934-015-0393-3</a>

Result language
angličtina
Original language name
Exacerbation of Substrate Toxicity by IPTG in Escherichia coli BL21(DE3) Carrying a Synthetic Metabolic Pathway
Original language description
Background: Heterologous expression systems based on promoters inducible with isopropyl-beta-D-1- thiogalactopyranoside (IPTG), e.g., Escherichia coli BL21(DE3) and cognate LacIQ/PlacUV5-T7 vectors, are commonly used for production of recombinant proteins and metabolic pathways. The applicability of such cell factories is limited by the complex physiological burden imposed by overexpression of the exogenous genes during a bioprocess. This burden originates from a combination of stresses that may includecompetition for the expression machinery, sidereactions due to the activity of the recombinant proteins, or the toxicity of their substrates, products and intermediates. However, the physiological impact of IPTG-induced conditional expression on the recombinant host under such harsh conditions is often overlooked. Results: The physiological responses to IPTG of the E.
Czech name
—
Czech description
—

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
—

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)