Eudiplozoon nipponicum (Heteronchoinea: Diplozoidae): insight into composition of E/S products and basic characterization of serpin

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F16%3A00088448" target="_blank" >RIV/00216224:14310/16:00088448 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Eudiplozoon nipponicum (Heteronchoinea: Diplozoidae): insight into composition of E/S products and basic characterization of serpin

Original language description

Two genes coding serpins, were expressed in E. coli system (BL21 strain) in combination with synthetic genes, optimised for this system, using pET-22B(+) expression vector. IPTG induced expression (30°C, overnight) was analyzed on SDS-PAGE and soluble recombinant serpins of E. nipponicum was partially purified using Ni-NTA agarose beads. Successful production of serpins was verified by mass spectrometry and Western blot using His-tag primary antibodies.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

EG - Zoology

OECD FORD branch

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Project

<a href="/en/project/GBP505%2F12%2FG112" target="_blank" >GBP505/12/G112: ECIP - European Centre of Ichtyoparasitology</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů