Systematic mapping of WNT-Frizzled interactions reveals functional selectivity by distinct WNT-Frizzled pairs

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F15%3A00082545" target="_blank" >RIV/00216224:14310/15:00082545 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1074/jbc.M114.612648" target="_blank" >http://dx.doi.org/10.1074/jbc.M114.612648</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1074/jbc.M114.612648" target="_blank" >10.1074/jbc.M114.612648</a>

Result language
angličtina
Original language name
Systematic mapping of WNT-Frizzled interactions reveals functional selectivity by distinct WNT-Frizzled pairs
Original language description
The seven transmembrane spanning receptors of the Class Frizzled (FZD1-10) are bound and activated by the WNT family of lipoglycoproteins, thereby inducing a complex network of signaling pathways. Yet the specificity of interaction between mammalian WNTand FZD proteins and the subsequent signaling cascade downstream of the different WNT FZD pairs has not been systematically addressed to date. In this report, we determine the binding affinities of various WNT proteins to different members of the FZD family by using biolayer interferometry and characterize their functional selectivity in a cellular system. Using purified WNT proteins, we show that different FZD cysteine rich domains (CRD) prefer to bind to distinct WNTs with fast onrates and slow off-rates.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
ED - Physiology
OECD FORD branch
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Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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