Determination of serum total protein by the Kjeldahl method

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F15%3A00082763" target="_blank" >RIV/00216224:14310/15:00082763 - isvavai.cz</a>
Result on the web
<a href="http://2015.svkprifuk.info/zbornik2015.pdf" target="_blank" >http://2015.svkprifuk.info/zbornik2015.pdf</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Determination of serum total protein by the Kjeldahl method
Original language description
Chromý et al. found in 2009, that albumin-calibrated analysis of serum total protein according to a candidate reference method, developed by Doumas working group in 1981, provide results with unacceptable positive bias. The simplest way to rectify this defect is the use of serum and/or plasma-based protein standards certified by the Kjeldahl method. We reviewed Kjeldahl methods adopted by laboratory medicine and selected for total protein a direct analysis on washed protein precipitates with trichloroacetic acid and verified its preliminary analytical performance parameters. We describe a final simplified analysis with modified still unit and digestion flask serving for both digestion and distillation and present its re-validation.
Czech name
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Czech description
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Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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