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Simplified direct Kjeldahl method suitable as a primary reference procedure for the determination of total protein in reference materials used in clinical chemistry

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F15%3A00108650" target="_blank" >RIV/00216224:14310/15:00108650 - isvavai.cz</a>

  • Result on the web

    <a href="http://www.cskb.cz/res/file/KBM-pdf/2015/2015-4/KBM-4-2015-Chromy-188.pdf" target="_blank" >http://www.cskb.cz/res/file/KBM-pdf/2015/2015-4/KBM-4-2015-Chromy-188.pdf</a>

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Simplified direct Kjeldahl method suitable as a primary reference procedure for the determination of total protein in reference materials used in clinical chemistry

  • Original language description

    Chromý et al. found in 2009, that albumin-calibrated analysis of serum total protein with biuret reagent according to a candidate reference method developed by Doumas working group in 1981, provide results with unacceptable positive bias, caused namely by endogeneous serum bilirubin and lipids. They also found, that a simplest way to rectify this defect, is the use of serum and/or plasma-based protein standards, commutable with patients samples, which are certified by the Kjeldahl method. In our foregoing two articles, (Chromý et al., 2015, and Vinklárková et al., 2015), we reviewed Kjeldahl methods adopted by laboratory medicine, selected a direct analysis on washed protein precipitates with trichloroacetic acid and verified its preliminary analytical performance parameters. In this article, we describe a simplified Kjeldahl analysis with modified digestion flask, now directly connectable to accordingly adapted Parnas and Wagner distillation unit, and present its re-validation. Simplified Kjeldahl method is suggested as a standard operating procedure for total protein in reference materials used in clinical chemistry

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>SC</sub> - Article in a specialist periodical, which is included in the SCOPUS database

  • CEP classification

  • OECD FORD branch

    10406 - Analytical chemistry

Result continuities

  • Project

  • Continuities

    S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2015

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Klinická biochemie a metabolismus

  • ISSN

    1210-7921

  • e-ISSN

  • Volume of the periodical

    23

  • Issue of the periodical within the volume

    4

  • Country of publishing house

    CZ - CZECH REPUBLIC

  • Number of pages

    5

  • Pages from-to

    188-192

  • UT code for WoS article

  • EID of the result in the Scopus database

    2-s2.0-84951044203