The use of flower-like gold nanoparticles in time-of-flight mass spectrometry
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F15%3A00082770" target="_blank" >RIV/00216224:14310/15:00082770 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
The use of flower-like gold nanoparticles in time-of-flight mass spectrometry
Original language description
Various types of gold nanoparticles (AuNPs) represent promising tools in many biomedical applications.[1-2] Here we report the first use of flower-like (FL) AuNPs as a mediator enhancing ionization in mass spectrometry (MS) of biomolecules. The main objective was to determine the effect of FL AuNPs on the ionization of biomolecules by surface assisted and matrix assisted laser desorption ionization (SALDI and MALDI) MS with time-of-flight analyzer. FL AuNPs alone or combined with cyano-4-hydroxycinnamicacid (CHCA) for MS measurement of peptides (500-3 500 m/z) were used. The comparison with classical CHCA matrix shows that FL AuNPs increase the ionization (~ 7.5 x) of peptides both alone and in combination with CHCA matrix. For ionization of higher mass peptides/proteins and those from mouse embryonic fibroblast (MEF) cells, FL AuNPs combined with CHCA and sinapinic acid (SA) were used.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
<a href="/en/project/EE2.3.20.0185" target="_blank" >EE2.3.20.0185: Centre for analysis and modeling of tissues and organs</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů