One point mutation can overturn sugar-binding specificity of lectin RS-IIL from plant pathogen Ralstonia solanacearum
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F15%3A00087734" target="_blank" >RIV/00216224:14310/15:00087734 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
One point mutation can overturn sugar-binding specificity of lectin RS-IIL from plant pathogen Ralstonia solanacearum
Original language description
A soil bacterium Ralstonia solanacearum is an aggressive phytopathogen which causes severe wilts of many economically important crops in tropical and subtropical regions. This pathogen produces two lectins: RSL and RS-IIL. Lectins are sugar-binding proteins different from sugar-specific antibodies or enzymes. They often play an important role in host-pathogen recognition and interactions. Lectin RS-IIL belongs to a so-called “PA-IIL family”, which includes also lectins PA-IIL (from P. aeruginosa), CV-IIL (from C. violaceum), BC2L-A and BC2L-C (from B. cenocepacia). These lectins are orthologs with significant sequential and structural similarities. Although they bind saccharides with similar stereochemistry, they differ in preferences towards these saccharides. This fact is caused by differences in three amino acids which are arranged in the so-called “specificity binding loop”. Adam et al. prepared three point mutants of the PA-IIL lectin to investigate the fine specificity.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů