Efficient non-enzymatic cleavage of Staphylococcus aureus plasmid DNAs mediated by neodymium ions

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F16%3A00087678" target="_blank" >RIV/00216224:14310/16:00087678 - isvavai.cz</a>
Alternative codes found
RIV/00216305:26310/16:PU119089
Result on the web
<a href="http://www.sciencedirect.com/science/article/pii/S0003269716300884" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0003269716300884</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.ab.2016.05.013" target="_blank" >10.1016/j.ab.2016.05.013</a>

Result language
angličtina
Original language name
Efficient non-enzymatic cleavage of Staphylococcus aureus plasmid DNAs mediated by neodymium ions
Original language description
Staphylococcus aureus plasmids are the main factor in the spreading of antibacterial resistance among bacterial strains that has emerged on a worldwide scale. Plasmids recovered from 12 clinical and food isolates of S. aureus were treated with 10 mM free lanthanide Nd3+ ions (non-enzymatic cleavage agent) in Hepes buffer (pH 7.5) at 70 °C. Topological forms of plasmids—closed circular (ccc), open circular (oc), and linear (lin)—produced by cleavage at different times were separated using pulsed-field agarose gel electrophoresis. The method is proposed to detect and differentiate several plasmids in the same bacterial strain according to their size.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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