ANALYSIS OF PLASMID DNA ISOLATED FROM STRAINS OF STAPHYLOCOCCUS AUREUS USING ARTIFICIAL NUCLEASES

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F13%3APU104111" target="_blank" >RIV/00216305:26310/13:PU104111 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
ANALYSIS OF PLASMID DNA ISOLATED FROM STRAINS OF STAPHYLOCOCCUS AUREUS USING ARTIFICIAL NUCLEASES
Original language description
Hydrolysis of nucleic acids mediated by artificial nucleases is very important goal in molecular biology, biotechnology and medicine. Some metal and lanthanide ions and their soluble or insoluble complexes (immobilized on solid particles) are attractiveas catalysts for DNA cleavage. The aim of this work was to use of neodymium ions for cleavage of plasmid DNAs. Plasmid DNAs originated from strains of Staphylococcus aureus isolated from clinical and food samples were treated by neodymium ions (Nd3+). Plasmid pUC19 was used as model molecule and linearized pUC19/EcoRI was used as the control of the presence of linear form. Cleavage of plasmid DNA was carried out at different temperatures in HEPES (0.05M) buffer. The reaction time was up to 4 hours. Thereaction was stopped with 0.5 M EDTA (pH 8.0). Plasmid DNA cleavage efficiency was evaluated by convert the supercoiled circular (ccc) form of plasmid DNA into nicked circular form (oc) and linear (lin) one and was monitored on the gel ac
Czech name
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Czech description
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Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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