MALDI TOF MS imaging of 3D neuroblastoma cell cultures
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F16%3A00087892" target="_blank" >RIV/00216224:14310/16:00087892 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
MALDI TOF MS imaging of 3D neuroblastoma cell cultures
Original language description
Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) has become a routine technique for analyte visualization across biological samples. In a single experiment, it enables imaging both exogenous and endogenous compounds, such as drugs, proteins and lipids. Additional great benefit of this approach is no need for molecule labelling, in comparison with the methods such as fluorescent microscopy, immunohistochemistry etc. In our study, 3D cell cultures SK-N-Be(2) and SH SY5Y were analyzed. The cell formations (spheroids) were embedded in gelatine, frozen, cryo-sectioned into thin slices and thawed to conductive glass slides. For uniform matrix coating, commercial iMatrixSpray sprayer was employed, its parameters were optimized and the results were compared with sublimation method. Developed protocols were applied to the analysis of spheroids treated by potential cancerostatics metaiodobenzylguanidine, perifosine and MK 2206.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů